A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

28.2K Views

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11:00 min

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June 22nd, 2010

DOI :

10.3791/1861-v

June 22nd, 2010

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Douglas Nam¹, Chih-Wen Ni², Amir Rezvan¹, Jin Suo², Klaudia Budzyn¹, Alexander Llanos¹, David G. Harrison¹, Don P. Giddens², Hanjoong Jo¹^,²^,³

¹Department of Medicine, Division of Cardiology, Emory University, ²Coulter Department of Biomedical Engineering, Georgia Tech and Emory University, ³Department of Bioinspired Science, Ewha Womans University

Summary

Chapters in this video

0:00

Title

0:42

Introduction

1:24

Partial Ligation of the Left Carotid Artery

4:21

Carotid Ultrasound Examination to Validate Disturbed Carotid Flow

6:28

Intimal RNA Isolation From Carotid

9:19

Representative Results

10:11

Conclusion

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