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Application of Stopped-flow Kinetics Methods to Investigate the Mechanism of Action of a DNA Repair Protein

DOI :

10.3791/1874-v

March 31st, 2010

March 31st, 2010

17,866 Views

1Molecular Biology and Biochemistry Department, Wesleyan University

Msh2-Msh6 is responsible for initiating repair of replication errors in DNA. Here we present a transient kinetics approach towards understanding how this critical protein works. The report illustrates stopped-flow experiments for measuring the coupled DNA binding and ATPase kinetics underlying Msh2-Msh6 mechanism of action in DNA repair.

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Stopped flow Kinetics Methods

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