JoVE Logo
Faculty Resource Center

Sign In

Development of automated imaging and analysis for zebrafish chemical screens.

DOI :

10.3791/1900-v

10:49 min

June 24th, 2010

June 24th, 2010

11,053 Views

1Pharmacology and Chemical Biology, University of Pittsburgh Drug Discovery Institute, 2Department of Microbiology and Molecular Genetics, University of Pittsburgh, 3Department of Pharmaceutical Sciences, University of Pittsburgh, 4Department of Chemistry, University of Pittsburgh

We report the development of a system for automated imaging and analysis of zebrafish transgenic embryos in multiwell plates. This demonstrates the ability to measure dose dependent effects of a small molecule, BCI, on Fibroblast Growth Factor reporter gene expression and provide technology for establishing high-throughput zebrafish chemical screens.

Tags

Automated Imaging

-- Views

Related Videos

article

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity

article

Semi-automated Optical Heartbeat Analysis of Small Hearts

article

Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos

article

Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry

article

Competitive Genomic Screens of Barcoded Yeast Libraries

article

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

article

In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina

article

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development

article

Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos

article

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved