JoVE Logo
Faculty Resource Center

Sign In

Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish

DOI :

10.3791/4203-v

7:50 min

July 16th, 2012

July 16th, 2012

13,792 Views

1Institute for Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe, Germany, 2Institute for Applied Informatics, Karlsruhe Institute of Technology, Karlsruhe, Germany

Here we describe a novel high-content chemically induced inflammation assay aiming at the identification of immune-modulatory bioactives. We have successfully combined automated microscopy with custom developed software scripts enabling automated quantification of the inflammatory response as well as further data processing, analysis, mining, and storage.

Tags

Drug Discovery

-- Views

Related Videos

article

Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation

article

Assays for the Identification of Novel Antivirals against Bluetongue Virus

article

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

article

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

article

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants

article

An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen

article

Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay

article

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

article

An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

article

Visualization of Biofilm Formation in Candida albicans Using an Automated Microfluidic Device

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved