JoVE Logo
Faculty Resource Center

Sign In

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

DOI :

10.3791/52063-v

7:45 min

November 8th, 2014

November 8th, 2014

16,977 Views

1Department of Biological Sciences, University of Notre Dame

The zebrafish is an excellent experimental organism to study vertebrate developmental processes and model human disease. Here, we describe a protocol on how to perform a manual high-throughput chemical screen in zebrafish embryos with a whole-mount in situ hybridization (WISH) read-out.

Tags

Manual Small Molecule Screen

-- Views

Related Videos

article

Generating Chimeric Zebrafish Embryos by Transplantation

article

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

article

Large Scale Zebrafish-Based In vivo Small Molecule Screen

article

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

article

High-throughput Yeast Plasmid Overexpression Screen

article

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

article

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

article

High Throughput Microinjections of Sea Urchin Zygotes

article

A Method of Permeabilization of Drosophila Embryos for Assays of Small Molecule Activity

article

Recovery of Adult Zebrafish Hearts for High-throughput Applications

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved