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Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

DOI :

10.3791/62999-v

6:02 min

March 22nd, 2022

March 22nd, 2022

6,426 Views

1Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, 2JST PRESTO, 3Graduate School of Chemical Sciences and Engineering, Hokkaido University

Microfluidic-based lipid nanoparticle (LNP) production methods have attracted attention in drug delivery systems (DDSs), including RNA delivery. This protocol describes the fabrication, LNP (siRNA-loaded LNP) production, and LNP evaluation processes using our original microfluidic device named iLiNP.

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SiRNA loaded Lipid Nanoparticles

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