The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow

41.7K Views

•

14:33 min

•

May 6th, 2010

DOI :

May 6th, 2010

•

Zaman Mirzadeh¹, Fiona Doetsch²^,³, Kazunobu Sawamoto⁴, Hynek Wichterle²^,⁵, Arturo Alvarez-Buylla¹

¹Department of Neurosurgery, The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco - UCSF, ²Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, ³Department of Neuroscience and Neurology, College of Physicians and Surgeons, Columbia University, ⁴Department of Developmental and Regenerative Biology, Nagoya City University Graduate School of Medical Sciences, ⁵Center for Motor Neuron Biology and Disease, College of Physicians and Surgeons, Columbia University

Summary

Explore More Videos

Subventricular Zone

Wholemount Staining

Lateral Ventricles

Germinal Region

Neural Stem Cells

Adult Neurogenesis

Wholemount Technique

Histological Analysis

Cytoarchitecture

Cellular Relationships

Neuroepithelium

Planar Polarization

Cerebrospinal Fluid Flow

Stem Cell Niche

Chapters in this video

0:00

Title

0:07

Introduction

1:02

Wholemount Dissection

4:14

Ependymal Flow Analysis Using Fluorescent Microbeads

6:41

Fixing and Immunostaining Wholemounts

10:58

Representative Images and Ependymal Flow Data

12:20

Conclusion

Related Videos

article

The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry

15.4K Views

article

Wholemount Immunohistochemistry for Revealing Complex Brain Topography

15.5K Views

article

Preparation of Acute Subventricular Zone Slices for Calcium Imaging

13.1K Views

article

Neonatal Subventricular Zone Electroporation

12.7K Views

article

Visualizing the Effects of a Positive Early Experience, Tactile Stimulation, on Dendritic Morphology and Synaptic Connectivity with Golgi-Cox Staining

17.9K Views

article

Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina

14.4K Views

article

Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey

9.8K Views

article

Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain

13.7K Views

article

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

14.1K Views

article

Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone

15.0K Views

Copyright © 2025 MyJoVE Corporation. All rights reserved