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13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement (Video) | JoVE

DOI :

10.3791/200017-v

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4:55 min

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May 19th, 2023

May 19th, 2023

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179 Views
1Program in Molecular Medicine, University of Massachusetts Chan Medical School, 2Cancer Center, University of Massachusetts Chan Medical School
* These authors contributed equally

Tags

Keywords 13C4 aspartate
13C5 glutamine
Stable Isotope Tracing
LC MS Analysis
SDH Activity Measurement
143B Human Osteosarcoma Cells
Cell Culture
Metabolite Extraction
Vacuum Concentration
Liquid Chromatography
Mass Spectrometry

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