November 13th, 2015
•Here, we present a modification of a previously reported method that allows for the isolation of high quality and purified mitochondria from smaller quantities of mouse skeletal muscle. This procedure results in highly coupled mitochondria that respire with high function during microplate based respirometirc assays.
Related Videos
Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
Mitochondrial Isolation from Skeletal Muscle
Purification of Progenitors from Skeletal Muscle
Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle
Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements
Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts
Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current
Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved