Generation of Cerebral Organoids from Primate-Induced Pluripotent Stem Cells (IPSCs)

To begin, wash the cultured iPSCs with Dulbecco's PBS or DPBS. And add one milliliter of proteolytic and collagenolytic mixture. Incubate the dish at 37 degrees Celsius for two minutes to detach the cells.

Next, add 1.5 milliliters of prewar