Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning
8.2K Views
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07:37 min
•
May 10th, 2019
DOI :
May 10th, 2019
•Lingling Xian1, Lionel Chia1,5, Dan Georgess2, Li Luo1, Shuai Shuai1, Andrew J. Ewald3,4, Linda M.S. Resar1,4,5,6
1Department of Medicine, Division of Hematology, Johns Hopkins University School of Medicine, 2Department of Natural Sciences, School of Arts & Sciences, Lebanese American University, 3Department of Cell Biology, Johns Hopkins University School of Medicine, 4Department of Oncology, Johns Hopkins University School of Medicine, 5Department of Pathology, Johns Hopkins University School of Medicine, 6Institute for Cell Engineering, Johns Hopkins University School of Medicine
Chapters in this video
0:04
Title
1:29
Genetic Engineering of Organoids by Viral Transduction
2:26
Seeding of Infected Organoid Fragments
3:44
Organoid Cryosection in Basement Membrane Matrix
5:32
Results: Efficient Viral Transduction of Intestinal Organoids
6:21
Conclusion
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