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Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks

DOI :

10.3791/54055-v

May 13th, 2016

May 13th, 2016

13,476 Views

1Department of Molecular and Cell Biology, University of California, Berkeley

Transgenic manipulations and genome editing are critical for functionally testing the roles of genes and cis-regulatory elements. Here a detailed microinjection protocol for the generation of genomic modifications (including Tol2-mediated fluorescent reporter transgene constructs, TALENs, and CRISPRs) is presented for the emergent model fish, the threespine stickleback.

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