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Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis

DOI :

10.3791/54313-v

9:38 min

October 16th, 2016

October 16th, 2016

15,287 Views

1Department of Evolution and Developmental Biology, Zoological Institute, University Innsbruck

We present transient transgenesis and gene knockdown in Ciona intestinalis, a chordate sister group to vertebrates, using microinjection and electroporation techniques. Such methods facilitate functional genomics in this simple invertebrate that features rudimentary characteristics of vertebrates, including notochord and head sensory epithelia, and many orthologs of human disease associated genes.

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Embryo Microinjection

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