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Imaging SH-SY5Y Cells by STED Microscopy and Subsequent Analysis of Mitochondrial Ultrastructure (Video) | JoVE

DOI :

10.3791/200327-v

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4:05 min

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June 30th, 2023

June 30th, 2023

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158 Views
1Center for Open Research Resources and Equipment, University of Connecticut, 2Department of Molecular and Cell Biology, University of Connecticut

Tags

Keywords STED Microscopy
Mitochondrial Ultrastructure
SH SY5Y Cells
LightBox Software
Confocal Acquisition Settings
STED Acquisition
Deconvolution
Trainable Weka Segmentation
Cristae
Image Analysis

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