Time-Lapse Imaging of Mitochondrial Transport Along Telencephalic Glutamatergic Neuron Axons
2:44
Cortical Neuron Mitochondrial Transport Analysis
4:17
Cortical Neuron Mitochondrial Morphology Analysis
5:32
Results: Representative Mitochondrial Transport and Morphology Analyses
6:47
Conclusion
Transcript
Mitochondrial dysfunction underlies many neurodegenerative diseases. Our protocol provides an important tool for examining mitochondrial dynamics in the axons, facilitating the study of neurological diseases involving axonal degeneration. By combi
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Impaired mitochondrial transport and morphology are involved in various neurodegenerative diseases. The presented protocol uses induced pluripotent stem cell-derived forebrain neurons to assess mitochondrial transport and morphology in hereditary spastic paraplegia. This protocol allows characterization of mitochondrial trafficking along axons and analysis of their morphology, which will facilitate the study of neurodegenerative disease.