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Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

DOI :

10.3791/54214-v

July 9th, 2016

July 9th, 2016

13,569 Views

1Virginia Tech Carilion Research Institute, 2Renovo Neural Incorporated

Mitochondrial visualization and analysis from mammalian brain tissue is a challenging task. Here, we describe how three dimensional (3D) reconstruction analysis from the serial block-face scanning electron microscopy (SBFSEM) can be used to gain insights on the morphological and volumetric analysis of this critical energy generating organelle.

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