High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies
2.6K Views
•
07:10 min
•
September 29th, 2023
DOI :
September 29th, 2023
•Sagar Raturi1, Huanyu Li1, Yung-Ning Chang2, Andreea Scacioc1, Tina Bohstedt1, Alejandra Fernandez-Cid1, Adam Evans1, Patrizia Abrusci1, Abilasha Balakrishnan1, Tomas C. Pascoa1, Didi He1, Gamma Chi1, Nanki Kaur Singh1, Mingda Ye1, Anna Li1, Leela Shrestha1, Dong Wang1, Eleanor P. Williams1, Nicola A. Burgess-Brown1, Katharina L. Dürr1, Vera Puetter2, Alvaro Ingles-Prieto3, David B. Sauer1
Chapters in this video
0:00
Introduction
2:08
Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High‐Throughput Protein Expression
3:18
Purification of Human Solute Carriers (SLCs) for Developing SLC Targeting Therapies
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