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Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cell Dissociation and Optimal Cryopreservation Stage Determination

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03:06 min

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November 3rd, 2023

DOI :

10.3791/200823-v

November 3rd, 2023

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Xinyue Bai*¹^,²^,³^,⁴^,⁵^,⁶^,⁷, Ting Zhang*¹^,²^,³^,⁴^,⁵^,⁶^,⁷, Xinyue Zhu¹^,²^,³^,⁴^,⁵^,⁶^,⁷, Xianyu Huang¹^,²^,³^,⁴^,⁵^,⁶^,⁷, Haiyun Liu¹^,²^,³^,⁴^,⁵^,⁶^,⁷, Xiaoyan Ding⁸, Mei Jiang¹^,²^,³^,⁴^,⁵^,⁶^,⁷, Xiaodong Sun¹^,²^,³^,⁴^,⁵^,⁶^,⁷

¹Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, ²National Clinical Research Center for Eye Diseases, ³Shanghai Clinical Research Center for Eye Diseases, ⁴Shanghai Key Clinical Specialty, ⁵Shanghai Key Laboratory of Ocular Fundus Diseases, ⁶Shanghai Engineering Center for Visual Science and Photomedicine, ⁷Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, ⁸Stem Cell Core Facility, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences

* These authors contributed equally

Transcript

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Human Embryonic Stem Cells

Retinal Pigment Epithelial Cells

Cell Dissociation

Cryopreservation

Cell Incubation

Microscopy Observation

Cell Suspension

EDU Incorporation

Fluorescence Microscopy

Proliferation Assay

From the series

Advancing Cryostorage of Human Stem Cell-Derived Retinal Pigment Epithelial Cells

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