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Enhanced 3D Confocal Imaging of Solvent-Cleared Human Heart Tissues

38 Views

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01:33 min

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June 28th, 2024

DOI :

10.3791/202012-v

June 28th, 2024

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1University of California Los Angeles (UCLA) Cardiac Arrhythmia Center, UCLA Health System, David Geffen School of Medicine at UCLA, 2Neurocardiology Program of Excellence, UCLA, 3Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, 4Crump Institute for Molecular Imaging, David Geffen School of Medicine at UCLA, 5Jonsson Comprehensive Cancer Center, David Geffen School of Medicine at UCLA

* These authors contributed equally

Transcript

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3D Confocal Imaging
Solvent cleared Heart Tissues
Paraformaldehyde fixed Tissue
Confocal Microscopy
Dehydration
Methanol Series
Dichloromethane
Immunolabeling
Tissue Preparation
Laser Scanning Confocal Microscope
Fluorescence Emission Spectra
Tile Scan
Z stack Images
Cardiac Neurons
Myocardial Innervation

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Comprehensive 3D Analysis of Human Hearts from Micro to Macro Scales
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