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DNA Stable-Isotope Probing (DNA-SIP)

DOI :

10.3791/2027-v

August 2nd, 2010

August 2nd, 2010

42,846 Views

1Department of Biology, University of Waterloo

DNA stable-isotope probing is a cultivation-independent method to identify and characterize active communities of microorganisms that are capable of utilizing specific substrates. Assimilation of substrate enriched in heavy isotope leads to incorporation of labelled atoms into microbial biomass. Density gradient ultracentrifugation retrieves labelled DNA for downstream molecular analyses.

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DNA SIP

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