JoVE Logo
Faculty Resource Center

Sign In

Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans

DOI :

10.3791/2288-v

12:10 min

February 27th, 2011

February 27th, 2011

13,257 Views

1Department of Pediatrics, The Children's Hospital of Philadelphia, 2Department of Pediatrics, University of Pennsylvania

Stable isotopic profiling by gas chromatography mass spectrometric analysis of intermediary metabolic flux is described in the nematode, Caenorhabditis elegans. Methods are detailed for assessing isotopic enrichment in carbon dioxide, organic acids, and amino acids following isotope exposure either during development on agar plates or during adulthood in liquid culture.

Tags

Stable Isotopic Profiling

-- Views

Related Videos

article

Generation of Stable Transgenic C. elegans Using Microinjection

article

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

article

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

article

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

article

Basic Caenorhabditis elegans Methods: Synchronization and Observation

article

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

article

Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory

article

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

article

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

article

Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved