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JoVE Journal

Medicine

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Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes

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07:32 min

May 25th, 2022

May 25th, 2022

1,259 Views

0:05

Introduction

0:40

Contractile Function Analysis of Adult Rat Cardiac Myocytes

4:20

Data Analysis of Contractile Function in Isolated Myocytes

6:18

Results: Contractile Function in Cardiac Myocytes in Response to Pressure Overload (PO) and Gene Transfer

6:59

Conclusion

Transcript

This approach detects changes in contractile function during the development of heart failure. The functional response in myocytes to neurohormones and potential therapeutic agents can also be screened. Positioning the myocyte may take practice bu

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A set of protocols are presented that describe the measurement of contractile function via sarcomere length detection along with calcium (Ca2+) transient measurement in isolated rat myocytes. The application of this approach for studies in animal models of heart failure is also included.

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