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Microinjection Method for Anopheles gambiae Embryos

DOI :

10.3791/62591-v

July 7th, 2021

July 7th, 2021

3,675 Views

1Department of Microbiology & Molecular Genetics, University of California, Irvine, 2Department of Molecular Biology & Biochemistry, University of California, Irvine

Microinjection techniques are essential to introduce exogenous genes into the genomes of mosquitoes. This protocol explains a method used by the James laboratory to microinject DNA constructs into Anopheles gambiae embryos to generate transformed mosquitoes.

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