Embryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus

5.3K Views

05:59 min

May 24th, 2020

DOI :

10.3791/61375-v

May 24th, 2020


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Embryo Microinjection

Chapters in this video

0:04

Introduction

0:51

Alignment of C. quinquefasciatus Pre-blastoderm Stage Embryos

1:46

Embryo Microinjection

3:32

Embryo Recovery and Hatching

4:08

Results: Survival and Mutation Rates of Embryos Injected with Single and Multiplexed gRNAs Targeting White

5:25

Conclusion

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