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Embryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus (Video) | JoVE

DOI :

10.3791/61375-v

5:59 min

May 24th, 2020

May 24th, 2020

4,723 Views

1Section of Cell and Developmental Biology, University of California, San Diego, 2Department of Entomology and Plant Patholog, Auburn University, 3Tata Institute for Genetics and Society, University of California, San Diego

This protocol describes microinjection procedures for Culex quinquefasciatus embryos that are optimized to work with CRISPR/Cas9 gene editing tools. This technique can efficiently generate site-specific, heritable, germline mutations that can be used for building genetic technologies in this understudied disease vector.

Tags

Embryo Microinjection

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