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Quantifying Synapses: an Immunocytochemistry-based Assay to Quantify Synapse Number

35.6K Views

18:11 min

November 16th, 2010

DOI :

10.3791/2270-v

November 16th, 2010


Transcript

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Chapters in this video

0:00

Title

1:17

Introduction

2:16

Preparing and Fixing Dissociated Purified Rat Retinal Ganglion Cells

5:33

Imaging

7:04

Image Analysis and Co-localized Puncta Quantification

9:18

Preparing and Fixing Brain Sections

12:39

Imaging of Tissue Sections

13:34

Representative Imaging and Synapse Assay Results

17:15

Conclusion

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