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Isolation and Primary Culture of Mouse Aortic Endothelial Cells

DOI :

10.3791/52965-v

December 19th, 2016

December 19th, 2016

28,645 Views

1Center for Molecular Medicine and Genetics, Wayne State University, 2Third Xiangya Hospital and the Institute of Vascular Disease and Translational Medicine, Central South University

The vascular endothelial cells play a significant role in many important cardiovascular disorders. This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment. Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology.

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Isolation

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