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Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

DOI :

10.3791/2387-v

11:32 min

December 4th, 2010

December 4th, 2010

14,859 Views

1Department of Biochemistry, University of Toronto

Here we describe an assay that employs the power of microinjection coupled with fluorescent in situ hybridization in order to accurately measure the nuclear export kinetics of mRNA in mammalian somatic cells.

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MRNA Nuclear Export Kinetics

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