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Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

DOI :

10.3791/50066-v

December 17th, 2012

December 17th, 2012

14,157 Views

1Department of Biochemistry, University of Toronto

Here we describe a method to visualize endoplasmic reticulum-associated mRNAs in mammalian tissue culture cells. This technique involves the selective permeabilization of the plasma membrane with digitonin to remove cytoplasmic contents followed by fluorescent in situ hybridization to detect either bulk poly(A) mRNA or specific transcripts.

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