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Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries

DOI :

10.3791/4140-v

8:05 min

June 27th, 2012

June 27th, 2012

25,441 Views

1Department of Cell Biology, Harvard Medical School

A protocol for preparation of robust, small-scale HeLa nuclear extracts is described. This protocol is valuable for assays that require use of small populations of cells, such as cells treated with drugs or RNAi. The method should be applicable to a wide variety of gene expression assays and other cell types, including patient cells.

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Small scale Nuclear Extracts

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