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Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

DOI :

10.3791/59797-v

August 6th, 2019

August 6th, 2019

5,848 Views

1Laboratory of RNA Biology, Institute of Molecular Genetics of the Czech Academy of Sciences, 2Department of Genetics and Microbiology, Faculty of Science, Charles University

Biogenesis of spliceosomal snRNAs is a complex process involving various cellular compartments. Here, we employed microinjection of fluorescently labelled snRNAs in order to monitor their transport inside the cell.

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Microinjection

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