Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging

41.3K Views

•

07:29 min

•

December 1st, 2011

DOI :

10.3791/2770-v

December 1st, 2011

•

¹Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, ²The Medical Research Council Mitochondrial Biology Unit, University of Cambridge , ³National Library of Medicine, National Institutes of Health, ⁴Massachusetts Institute of Technology, ⁵William Fremd High School, ⁶University of Virginia , ⁷Duke University , ⁸Yale University, ⁹University of Notre Dame , ¹⁰Washington University in St. Louis , ¹¹Bioinformatics and Computational Biosciences Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, ¹²Thomas Jefferson High School for Science and Technology

Summary

Explore More Videos

HIV

Molecular Structures

Cryo Electron Tomography

Automated Sub tomogram Averaging

HIV Envelope Glycoproteins

CD4 T cells

Immune System

Acquired Immunodeficiency Syndrome AIDS

HIV Infection Pathway

HIV Research

HIV Vaccine

Cryo electron Tomography

3D Image Processing

Viral Membrane Proteins

Chapters in this video

0:05

Title

1:17

Prepare Vitrified Virus Sample

2:39

Loading Samples into the Transmission Electron Microscope

3:37

Acquiring Cryo-electron Microscopy Data

4:52

Converting Microscopy Data into Structural Information that can be Visualized

6:19

Representative Results and Conclusion

Related Videos

13:08

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

16.3K Views

09:53

Specific Marking of HIV-1 Positive Cells using a Rev-dependent Lentiviral Vector Expressing the Green Fluorescent Protein

12.8K Views

11:10

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

12.1K Views

12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

17.3K Views

07:31

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy

42.0K Views

10:49

In vitro Uncoating of HIV-1 Cores

15.6K Views

11:45

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

12.1K Views

05:46

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

17.6K Views

08:29

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

12.0K Views

07:10

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

14.2K Views