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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

DOI :

10.3791/62383-v

8:16 min

March 19th, 2021

March 19th, 2021

3,809 Views

1Structural and Computational Biology Unit, European Molecular Biology Laboratory, 2Electron Microscopy Core Facility, European Molecular Biology Laboratory, 3Imaging Centre, European Molecular Biology Laboratory

The increasing demand for large-scale data collection in cryogenic electron tomography requires high-throughput image acquisition routines. Described here is a protocol that implements the recent developments of advanced acquisition strategies aimed at maximizing the time-efficiency and throughput of tomographic data collection.

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Optimization

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