JoVE Logo
Faculty Resource Center

Sign In

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

DOI :

10.3791/63923-v

8:55 min

July 12th, 2022

July 12th, 2022

3,571 Views

1Electron Bio-Imaging Centre, Diamond Light Source Ltd, Harwell Science & Innovation Campus, 2Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, 3Chinese Academy of Medical Sciences Oxford Institute, University of Oxford

The present protocol describes high-resolution cryo-electron tomography remote data acquisition using Tomo5 and subsequent data processing and subtomogram averaging using emClarity. Apoferritin is used as an example to illustrate detailed step-by-step processes to achieve a cryo-ET structure at 2.86 Å resolution.

Tags

Cryo electron Tomography

-- Views

Related Videos

article

Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue

article

Evaluation of Lipid Droplet Size and Fusion in Bovine Hepatic Cells

article

Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures

article

Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

article

A Precise and Quantifiable Method for Collecting Hemolymph from Small Arthropods

article

Assembly and Operation of a Cooling Stage to Immobilize C. elegans on Their Culture Plates

article

Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons

article

A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

article

Generation and Manipulation of Rat Intestinal Organoids

article

Author Spotlight: The Production of Recombinant Proteins

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved