JoVE Logo
Faculty Resource Center

Sign In

Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography

DOI :

10.3791/62351-v

9:06 min

November 20th, 2021

November 20th, 2021

3,302 Views

1Central European Institute of Technology, Masaryk University

We present a protocol for lamella preparation of plunge frozen biological specimens by cryo-focused ion beam micromachining for high-resolution structural studies of macromolecules in situ with cryo-electron tomography. The presented protocol provides guidelines for the preparation of high-quality lamellae with high reproducibility for structural characterization of macromolecules inside the Saccharomyces cerevisiae.

Tags

Preparation

-- Views

Related Videos

article

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

article

Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

article

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

article

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

article

Analysis of Fluorescent-Stained Lipid Droplets with 3D Reconstruction for Hepatic Steatosis Assessment

article

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

article

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

article

Author Spotlight: Peptidome Extraction from Small Extracellular Vesicles Isolated from Bone Marrow-Derived Macrophages

article

Author Spotlight: Advancing Biotherapeutic Mass Calculation by Introducing mAbScale, a Python-Based Desktop Application

article

Author Spotlight: A Novel Method for Comprehensive Cell Component Analysis of Cerebral Blood Clots

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved