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Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles

DOI :

10.3791/3171-v

11:08 min

April 8th, 2012

April 8th, 2012

34,389 Views

1System Biosciences

Lentiviral expression vectors are the most effective vehicles for stably expressing different effector molecules or reporter constructs in dividing and non-dividing mammalian cells and whole organisms. Here we provide a protocol on how to package lentivector expression constructs in pseudoviral particles and to transduce target cells using the pseudoviral particles.

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HIV based Lentivector Expression Constructs

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