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In vitro Uncoating of HIV-1 Cores

DOI :

10.3791/3384-v

November 8th, 2011

November 8th, 2011

15,395 Views

1Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine

Uncoating is an essential step in the early phase of the HIV-1 life cycle and is defined as the disassembly of the capsid shell and the release of the viral ribonucleoprotein complex (vRNP). Here, we demonstrate techniques for isolating intact cores from HIV-1 virions and for quantifying their uncoating in vitro.

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In Vitro Uncoating

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