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Expansion of Human Peripheral Blood γδ T Cells using Zoledronate

DOI :

10.3791/3182-v

September 9th, 2011

September 9th, 2011

25,949 Views

1Department of Immunotherapeutics (Medinet), University of Tokyo Hospital, 2MEDINET Co., Ltd

A method to expand γδ T cells from peripheral blood mononuclear cells (PBMC) is described. PBMC-derived γδ T cells are stimulated and expanded using zoledronate and interleukin-2 (IL-2). Large scale expansion of γδ T cells can be applied to autologous cellular immunotherapy of cancer.

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Human Peripheral Blood

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