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Generation of Human Alloantigen-specific T Cells from Peripheral Blood

DOI :

10.3791/52257-v

November 21st, 2014

November 21st, 2014

12,694 Views

1Department of Pathology, University of California, San Diego

This article describes a method for the generation and propagation of human T cell clones that specifically respond to a defined alloantigen. This protocol can be adapted for cloning human T cells specific for a variety of peptide-MHC ligands.

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Human T Cells

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