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Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

2.8K Views

11:52 min

January 27th, 2023

DOI :

10.3791/64698-v

January 27th, 2023


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Osteoclast Differentiation

Chapters in this video

0:04

Introduction

0:56

Isolation of Peripheral Blood Mononuclear Cells (PBMCs) from Whole Blood

2:34

Enrichment of CD14+ Monocytes from PBMCs

4:20

Osteoclast Differentiation In Vitro

5:46

TRAP Staining for Osteoclasts

7:10

Actin Ring Fluorescent Staining

9:40

Results: Differentiation of Osteoclasts and Assessing the Function of Mature Osteoclasts

11:12

Conclusion

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