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Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein

DOI :

10.3791/52427-v

March 9th, 2015

March 9th, 2015

12,006 Views

1Programme in Molecular Structure and Function, Hospital for Sick Children, 2Department of Biochemistry, University of Toronto, 3Department of Physiology, University of Toronto

Described here is a rapid and effective procedure for functional reconstitution of purified wild-type and mutant CFTR protein that preserves activity for this chloride channel, which is defective in Cystic Fibrosis. Iodide efflux from reconstituted proteoliposomes mediated by CFTR allows studies of channel activity and the effects of small molecules.

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Functional Reconstitution

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