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RNA Secondary Structure Prediction Using High-throughput SHAPE

DOI :

10.3791/50243-v

May 31st, 2013

May 31st, 2013

30,354 Views

1RT Biochemistry Section, HIV Drug Resistance Program, Frederick National Laboratory for Cancer Research

High-throughput selective 2' hydroxyl acylation analyzed by primer extension (SHAPE) utilizes a novel chemical probing technology, reverse transcription, capillary electrophoresis and secondary structure prediction software to determine the structures of RNAs from several hundred to several thousand nucleotides at single nucleotide resolution.

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