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Ex Vivo Organotypic Corneal Model of Acute Epithelial Herpes Simplex Virus Type I Infection

12.6K Views

07:55 min

November 3rd, 2012

DOI :

10.3791/3631-v

November 3rd, 2012


Transcript

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Ex Vivo Organotypic Corneal Model

Chapters in this video

0:00

Title

0:47

Introduction

2:08

Materials

2:36

Excision of the Corneoscleral Button

3:29

Introduction of Cornea into Organotypic Culture

4:22

Infection with HSV-1 and Treatment with Experimental Drugs

5:25

Medium for Plaque Assays

5:45

DNA, RNA, Protein, or Cells from Corneal Epithelium

6:48

Tissue Samples for Immunohistochemistry or Immunofluorescence

7:23

Conclusion

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