JoVE Logo
Faculty Resource Center

Sign In

Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy

DOI :

10.3791/3642-v

December 15th, 2011

December 15th, 2011

12,992 Views

1School of Chemistry, Food and Pharmacy, The University of Reading, 2Department of Nutritional Sciences, The University of Reading

A progressive colonization procedure is described to further assess its impact on the host hepatic metabolism. Colonization is monitored non invasively by evaluating the urinary excretion of microbial co-metabolites by NMR-based metabolic profiling while hepatic metabolism is assessed by High Resolution Magic Angle Spinning (HR MAS) NMR profiling of intact biopsy.

Tags

Hepatic Metabolic Changes

-- Views

Related Videos

article

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

article

Colonization of Euprymna scolopes Squid by Vibrio fischeri

article

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

article

Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae

article

Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

article

Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes

article

Selective Harvesting of Marginating-hepatic Leukocytes

article

A Murine Model of Group B Streptococcus Vaginal Colonization

article

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

article

Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved