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Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

DOI :

10.3791/3763-v

13:59 min

June 14th, 2012

June 14th, 2012

11,112 Views

1Institute for Cell and Molecular Biosciences, University of Newcastle, United Kingdom

The kinetochore is where the SAC initiates its signal monitoring the mitotic segregation of the sister chromatids. A method is described to visualize the recruitment and turnover of one of the kinetochore proteins and its coordination with the chromosome motion in Drosophila embryos using a Leica laser scanning confocal system.

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Mitotic Checkpoint

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