JoVE Logo
Faculty Resource Center

Sign In

A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels

DOI :

10.3791/3850-v

April 24th, 2012

April 24th, 2012

14,148 Views

1Department of Pharmacology, Physiology & Neuroscience, University of South Carolina, School of Medicine

A real-time screening procedure for identifying drugs that interact with G protein-gated inward rectifier K+ (GIRK) channels is described. The assay utilizes membrane potential-sensitive fluorescent dyes to measure GIRK channel activity. This technique is adaptable for use on a number of cell lines.

Tags

Fluorescent Screening Assay

-- Views

Related Videos

article

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

article

High-throughput Screening and Biosensing with Fluorescent C. elegans Strains

article

Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method

article

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

article

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

article

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

article

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters

article

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

article

Evaluation of Zebrafish Kidney Function Using a Fluorescent Clearance Assay

article

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved