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A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels

DOI :

10.3791/3850-v

April 24th, 2012

April 24th, 2012

14,144 Views

1Department of Pharmacology, Physiology & Neuroscience, University of South Carolina, School of Medicine

A real-time screening procedure for identifying drugs that interact with G protein-gated inward rectifier K+ (GIRK) channels is described. The assay utilizes membrane potential-sensitive fluorescent dyes to measure GIRK channel activity. This technique is adaptable for use on a number of cell lines.

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Fluorescent Screening Assay

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