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Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

DOI :

10.3791/3899-v

May 26th, 2013

May 26th, 2013

10,711 Views

1Department of Microbial and Environmental Genomics, The J. Craig Venter Institute

Single Virus Genomics (SVG) is a method to isolate and amplify the genomes of single virons. Viral suspensions of a mixed assemblage are sorted using flow cytometry onto a microscope slide with discrete wells containing agarose, thereby capturing the virion and reducing genome shearing during downstream processing. Whole genome amplification is achieved using multiple displacement amplification (MDA) resulting in genomic material that is suitable for sequencing.

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Isolation

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