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Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

DOI :

10.3791/3925-v

October 22nd, 2012

October 22nd, 2012

15,980 Views

1Department of Medicine, Vanderbilt University School of Medicine, 2Departments of Medicine and Immunology, Roswell Park Cancer Institute, 3Department of Medicine, University at Buffalo School of Medicine

NADPH oxidase is the major source of reactive oxygen species (ROS) in phagocytes. Because of the ephemeral nature of ROS, it is difficult to measure and monitor ROS levels in living animals. A minimally invasive method for serial quantification of ROS in living mice is described.

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