S'identifier

JoVE Journal

Immunology and Infection

Un abonnement à JoVE est nécessaire pour voir ce contenu.

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

16.1K Views

10:42 min

October 22nd, 2012

October 22nd, 2012

16,100 Views

0:05

Title

2:09

Animals Models: Intratracheal Administration of Zymosan

4:07

Animal Models: Cecal Ligation and Puncture (CLP)

5:15

Animal Models: Administration of Oral Carbon Tetrachloride

5:52

Acquiring Bioluminescent Images

6:55

Data Analysis using Region of Interest

7:45

Detection of NADPH Oxidase Mediated ROS Production

9:57

Conclusion

NADPH oxidase is the major source of reactive oxygen species (ROS) in phagocytes. Because of the ephemeral nature of ROS, it is difficult to measure and monitor ROS levels in living animals. A minimally invasive method for serial quantification of ROS in living mice is described.

Nous utilisons des cookies afin d'améliorer votre expérience sur notre site web.

En continuant à utiliser notre site ou en cliquant sur le bouton ''continuer'', vous acceptez l'utilisation de cookies.