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Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures

DOI :

10.3791/3951-v

October 28th, 2012

October 28th, 2012

11,529 Views

1Department of Engineering, University of California, Merced

Despite ongoing efforts to transition cultures to feeder-free conditions, the derivation and culture of human embryonic stem cells (hESC) remain largely dependent on co-cultures with mouse embryonic feeders (MEFs). Here, we show a novel methodology for rapidly removing feeders from hESC cultures prior to experimentation.

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Fibroblast Removal

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