Results: Analysis of the Generated Primary Murine Melanocyte and Fibroblast Cultures
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Transcript
This protocol describes a rapid and simple method to generate primary melanocyte and fibroblast cultures from mice. Because this technique does not require that the epidermis and dermis are separated, it can be performed quickly and consistently w
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This protocol outlines a rapid method to simultaneously generate melanocyte and fibroblast cultures from the skin of 0-4 day old mice. These primary cultures can be maintained and manipulated in vitro to study a variety of physiologically relevant processes, including skin cell biology, pigmentation, wound healing and melanoma.