JoVE Logo
Faculty Resource Center

Sign In

Rapid Generation of Primary Murine Melanocyte and Fibroblast Cultures

DOI :

10.3791/59468-v

June 26th, 2019

June 26th, 2019

10,419 Views

1Department of Cancer Biology and Genetics, The Ohio State University, 2Department of Molecular Genetics, The Ohio State University

This protocol outlines a rapid method to simultaneously generate melanocyte and fibroblast cultures from the skin of 0-4 day old mice. These primary cultures can be maintained and manipulated in vitro to study a variety of physiologically relevant processes, including skin cell biology, pigmentation, wound healing and melanoma.

Tags

Keywords Primary Melanocyte Culture

-- Views

Related Videos

article

A Mouse Model of Fatigue Induced by Peripheral Irradiation

article

A Rapid Filter Insert-based 3D Culture System for Primary Prostate Cell Differentiation

article

Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry

article

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

article

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

article

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

article

Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

article

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

article

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

article

Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved